Collection Instructions


Collection Method


Kit Requirements

11 x 5ml Saliva

Turn Around Time

3-5 Business Days Turnaround times are estimates


Sample Report


Many women experience symptoms related to their menstrual cycle.  Assessing progesterone and estradiol levels throughout a 28 day cycle can help ascertain whether a deficiency or excess of these hormones are present in the three phases of the menstrual cycle (follicular, ovulatory and luteal phases).

The salivary 28 day hormone test is a comprehensive assessment of (E2) estradiol, (P4) progesterone, and testosterone over a 28 day period of a female’s regular menstrual cycle. Hormone imbalances reveal the rhythm of hormones and their fluctuations which can help identify the key causes of disorders such as premenstrual syndrome (PMS), infertility, and menstrual irregularities. This hormonal test analyses 11 saliva samples of a 28-day period. Saliva samples for the most accurate test results are collected on days: 3, 5,8,11,12,14,16,18,20,23 and 28.

Hormonal balance is the key to optimal health. Research has long shown that fluctuating levels of estradiol, progesterone, and testosterone play a major role in a woman´ s overall health affecting a variety of conditions including menstrual cycles, sleep patterns, menopause, infertility PCOS, PMS, breast cancer and endometriosis.

Saliva hormone testing provides a thorough analysis of estradiol and progesterone over a full 28 day cycle and testosterone is measured on day 11. By utilising 11 saliva samples for analysis, the relationship and balance of these essential hormones are analysed more precisely through time. The levels of estradiol and progesterone, as well as the ratio between the two, are clearly graphed for easy reference and patient education. This tool serves as a useful diagnostic tool in clinical practice to help practitioners with treatment of chronic gynaecologic disorders.


  • Estradiol (E2) on Day 3, 5, 8, 11, 12, 14, 16, 18, 20, 23, 28
  • Progesterone on Day 3, 5, 8, 11, 12, 14, 16, 18, 20, 23, 28
  • Testosterone on Day 11

Test Method

EIA (Enzyme immunoassay)

Common Conditions

  • Other Health Conditions
    • Amenorrhea
    • Dysmenorrhea
    • Endometriosis
    • Irregular periods
    • Menopause
  • Reproductive Health
    • Infertility
    • Polycystic ovaries
    • Premenstrual Syndrome

Notice To Patients

NutriPATH practices in the usual practitioner-referral system for pathology laboratories. Patients are highly recommended to seek the supervision and guidance of a qualified healthcare practitioner for the interpretation of any lab results and associated information. NutriPATH can offer assistance in locating a suitable practitioner.

Find a Practitioner

How it works

Step 1

Order test

Your referring practitioner will place this order via their online portal.

Step 2

Collect Samples

A test kit will be dispatched to you and you can conveniently collect your sample in the comfort of your home.

Step 3

Return Samples

Return samples for analysis via the provided courier packaging.

Patient resources

Our highly skilled and qualified team have created a service where patients can easily gain access to a wide range of pathology testing options.

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Practitioner resources

As a leader in the industry, practitioners are able to trust in our ability to provide them with the technology they need to adequately test their clients and patients.

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